Detect and Eliminate Mycoplasma Contamination from your Valuable Cell Culture Samples
One challenge in dealing with mammalian cell culture is contamination due to mycoplasma.
These simple bacteria can infect cell cultures and alter a variety of cellular characteristics and functionalities (metabolism, morphology, proliferation etc.) often leading to experimental artifacts and cell loss. It is essential to detect for the presence of mycoplasma in cell cultures and remove it effectively, without compromising cell viability.
MP Biomedicals is pleased to announce the launch of new kit for high sensitivity and rapid detection of mycoplasma: Myco-Visible Mycoplasma LAMP Detection Kit.
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High Sensitivity: Requires as little as 10 16S rRNA copies or 10 fg (femtogram) of Mycoplasma genomic DNA per reaction.
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Rapid and Comprehensive: Detect Mycoplasma spp in 45 mins
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No Special Instrument Required: Direct detection with just a dry bath
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Easy to Use: Innovative colorimetric detection system – vivid color change
Other kits for high sensitivity detection of mycoplasma are the Myco-Sniff Mycoplasma PCR Detection Kit, and Myco-Sniff-Valid Mycoplasma PCR Detection Kit. Both kits simplify testing and detection of mycoplasma contamination in cell culture, ensuring:
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High sensitivity (as low as 10 CFU/mL)
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Wide detection range of mycoplasma species
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Premixed components for ease-of-use
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Quality control with inclusion of positive and negative controls
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High specificity, with no interference of animal or bacterial DNA
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Fast detection speed – as little as 3 hours
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Elimination of cross-contamination with inclusion of 8-MOP
The time-tested Hoechst Staining Kit for mycoplasma detection offers the following advantages:
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Reliable: Use of the Hoechst fluorescent stain method cited by the Tissue Culture Association (TCA procedure no. 75361)
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Efficient: It specifically and selectively binds to the minor grooves of DNA
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Versatile: In situ detection of mycoplasma and other prokaryotic organisms
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Rapid: Takes less than 2 hours
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Complete: Stain, diluent, and mounting medium with controls included in the kit
Once Mycoplasma has been detected, treat infected cell cultures with Mycoplasma Removal Agent (MRA). MRA is the most reliable solution for mycoplasma removal and prevention, ensuring quality results (as shown in the figure), including:
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Elimination of multiple mycoplasma species within one week using the lowest dosage
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Prevention of culture recontamination at 0.1µg/mL
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Maintenance of cell viability
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Compatibility with most mammalian cell lines
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Sterility and low cytotoxicity
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Citation and recognition in 550+ scientific publications
Myco-Out Mycoplasma Removal Spray
- Easy to Use: User-friendly, ready-to-use spray, eliminating the need for tedious methods like fumigation and alcohol wiping.
- Fast and Non-Toxic: The mycoplasma-specific peptide surfactant derived from Bacillus subtilis works in just 5 – 10 minutes without harming cells.
- Highly Effective: Efficacy studies confirm its effectiveness in removing Mycoplasma from surfaces like laminar flow cabinets coated with Mycoplasma-positive HeLa cell culture.
- Broad Antimicrobial Spectrum: It eliminates Mycoplasma and inactivates various microorganisms, including bacteria, viruses, and fungal spores.
Myco-Visible Mycoplasma Rapid Test Kit
Detects mycoplasma contamination with a sensitivity as low as 10 CFU/mL or 10 fg of genomic DNA.
Delivers results in under an hour, with positive results visible in less than 5 minutes on an immunochromatographic test device.
Effective against a wide range of mycoplasma species, including common ones like Mycoplasma orale and Mycoplasma hyorhinis, as well as less common species.
Simple to perform without the need for special technical expertise or equipment, making it ideal for routine cell culture monitoring.
Myco-Visible Mycoplasma LAMP Detection Kit
No Special Instrument Required: Direct detection with just a dry bath
Rapid and Comprehensive: Detects Mycoplasma spp in 60 minutes
Sensitive: Requires as little as 10 16s rRNA copies or 10 fg of Mycoplasma genomic DNA per reaction
Easy to use: Innovative colorimetric detection system - vivid color change.
Mycoplasma removal agent (MRA)
Eliminates mycoplasma contamination from cell cultures. Broad spectrum activity. Effective at low concentration. Proven low in cytotoxicity