DNA extraction of farm field soil with SPINeasy DNA Kit for Soil (Cat. No. 116530050)
Growing problems with farm field soil samples
The farm field soil sample is rich in both biological contents from microbials and small living organisms and chemical content from fertilizers and additives. The DNA extraction in the study of the quality of farm soil may be disrupted by unwanted contaminants and inhibitors that will affect both the quality and quantity for the DNA extracted.
MP Biomedicals shared on how SPINeasy DNA Kit for Soil (Cat. No. 116530050)can help to simplify the DNA extraction 's process, improve on the quality and quantity of the DNA extracted which will improve with the qPCR result downstream.
Overview
Sample Name:
Farm field Soil
Sample Type:
Featured Materials:
SPINeasy® DNA Pro Kit for Soil
High Performance kit for isolation of bacterial, fungal, plant and animal genomic DNA from soil and other environmental samples
Methods
Sample was prepared in order of protocol in accordance to product guidelines found here.
Outcome
Trial results data
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100g of soils were inoculated with same amount of fungal spores.
DNA was isolated from soil samples using soil DNA extraction kits by column membrane method and glass milk method respectively.
The results of qPCR assay by two methods agree well.
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Conclusion
Key takeaways:
The protocol is simple and easy to follow. In the study, a similar amount of fungal spores was added to control group. DNA was extracted from both the control and test groups with SPINeasy DNA kit for Soil and then analysed through qPCR. The similar CT values in both groups indicated that SPINeasy DNA extraction kit achieved high recovery and high purity of DNA.
Looking To Try Our Extraction Kits?MP Biomedicals is now offering Free Trial Pack Application for the SPINeasy® DNA Pro Kit for Soil (5 Preps) to any valid research scientists. |
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