SPINeasy 96-Well DNA Kit for Blood

SPINeasy 96-well DNA Kit for Blood is designed specifically for high-throughput genomic DNA (gDNA) extraction that caters for 96 samples and provides high quality gDNA product. This kit is developed based on spin column purification technology that enables convenient isolation of gDNA from a variety of blood sources using either centrifugation or vacuum manifold method. The SPINeasy 96-well DNA Kit for Blood can readily process fresh and frozen human whole blood preserved in different anticoagulants (EDTA, sodium citrate, and heparin). The purification ensures rapid and efficient DNA isolation, with proteins and salts being efficiently removed using our specialized wash buffers. Provided in the Kit, the 96-well spin column plate and kit buffers are designed to deliver gDNA extract of high yield and purity that is compatible with downstream applications such as long fragment PCR, real time PCR, restriction enzyme digestion, and sequencing.

Figure 1: SPINeasy 96-well DNA Kit for Blood offers excellent linearity in extraction performance regardless of extraction methods used. Both vacuum manifold and centrifuge methods consistently provide high yield and good purity of gDNA across blood samples preserved in different anticoagulants. DNA extraction of different individual EDTA blood samples were repeated in duplicates while citrate and heparin blood samples were extracted in single replicate using both vacuum manifold and centrifuge methods. No significant yield difference is observed between vacuum manifold and centrifuge methods.
EDTA: Blood preserved with EDTA anticoagulant, C: Blood preserved with sodium citrate anticoagulant, H: Blood preserved with heparin anticoagulant.

Figure 2: SPINeasy 96-well DNA Kit for Blood demonstrates a more consistent yield across wells and generally provides slightly higher yield, better purity ratios and DIN values as compared to the competitor kits (Q and N).

Figure 3: Both SPINeasy 96-well DNA Kit for Blood and SPINeasy DNA Kit for Blood (Cat. No. 116552050) have comparable performance in extracting blood DNA. E: Blood preserved with EDTA anticoagulant, C: Blood preserved with sodium citrate anticoagulant, H: Blood preserved with heparin anticoagulant.  

Figure 4.  gDNA extracted from SPINeasy 96-well DNA Kit for Blood successfully amplified gene fragments ranging from 106 bp to 5778 bp. E: Blood  preserved with EDTA anticoagulant, C: Blood preserved with sodium citrate anticoagulant, H: Blood preserved with heparin anticoagulant, PTC: Positive Control, NTC: Negative Control.

Figure 5. gDNA extracted from SPINeasy 96-well DNA Kit for Blood is suitable for downstream detection and quantification by qPCR.

 

 

 

 

 

Application Notes

The SPINeasy 96-Well DNA Kit for Blood was designed for the isolation of DNA from whole blood.

Usage Statement

Research Use Only (RUO)

Key Applications

Long fragment PCR, real time PCR, restriction enzyme digestion, sequencing