Soil samples are a complex environment characterized by large variations of microbial load and diversity that prove to be challenging to analyze due to the presence of some inhibitory compounds such as humic acid, heavy metals, and other aromatic components.

SPINeasy® DNA Kit for Microbiome employs a novel method for isolating microbial genomic DNA from various samples. Due to its highly effective lysis capability and silica-membrane spin-column technology, the SPINeasy® DNA Kit for Microbiome can be used for bacterial, body fluids and environmental samples such as soil and stool.

The SPINeasy® DNA/RNA Kit for Soil allows simultaneous isolation of pure microbiome genomic DNA and RNA from challenging soil types, including those with low biomass or those that are highly contaminated. The isolated nucleic acid products showed no contaminants and were immediately ready for use in downstream applications, including qPCR and RT-qPCR without the need of further inhibitor removal step.

SPINeasy® DNA Kit for Yeast is a high-performance genomic DNA (gDNA) extraction kit which is developed based on silica-membrane spin-column technology. This kit enables quick isolation of gDNA from yeast cells, typically in less than 30 minutes. 

SPINeasy® DNA Kit for Tissue (with Lysing Matrix) is a high-performance genomic DNA (gDNA) extraction kit which combines mechanical, chemical and enzymatic disruption of tissue samples in 30 minutes. The addition of proteinase K allows a more efficient and complete digestion of any tissue type. Homogenizing time is greatly reduced while substantially increasing the gDNA yield and purity. The kit is validated for tissue samples of fatty, tough, and fibrous samples, and suitable for downstream applications such as PCR and qPCR.

SPINeasy® DNA Kit for Tissue (without Lysing Matrix) is a high-performance genomic DNA (gDNA) extraction kit which combines silica column binding and enzymatic disruption of tissue samples in 60 minutes. The addition of proteinase K allows a more efficient and complete digestion of any tissue type. Homogenizing time is greatly reduced while substantially increasing the gDNA yield and purity. The kit is validated for tissue samples of fatty, tough, and fibrous samples, and suitable for downstream applications such as PCR and qPCR.

• Efficient process eliminates the requirement for enzymatic treatment and DNase I digestion, saving time.

• Superior yield for both DNA and RNA.

• Streamlined extraction with only one solvent and one additional binding buffer.

• Compatible with both fresh and frozen whole blood samples, addressing diverse research needs.

• Employing a 96-well DNA binding plate with superior binding capacity.

• Achieving high-yield genomic DNA extraction from various tissue samples, including tough, fibrous, and fatty tissues.

• Extracting gDNA with molecular weight of >50 kb with high purity, suitable for most downstream applications.

• Simple extraction protocol requiring no sophisticated equipment - less than 40 minutes of hands-on time.

• Designed in 96-well spin plate format, allowing for high throughput and rapid sample processing.

• Enables efficient lysis of microbial population present in complex soil samples, including bacteria, fungi, viruses, and protists; ensuring comprehensive genomic DNA extraction.

• Compatible with various challenging soil types, including those with low biomass or high contamination levels.

• Streamlined workflow reduces processing time, enabling faster and convenient isolation of pure microbial genomic DNA in 90 minutes.

The SPINeasy® DNA/RNA Kit for Feces allows simultaneous isolation of pure microbiome genomic DNA and RNA from challenging feces types irrespective of their composition. Isolated samples showed no contaminants and were immediately ready for use in downstream applications, including qPCR and RT-qPCR without the need of further inhibitor removal step.