The SPINeasy RNA Kit for Blood is an advanced RNA extraction solution leveraging state-of-the-art silica-membrane spin-column technology. Specifically designed for the isolation of RNA from whole blood preserved in various anticoagulants, this kit streamlines the process to under 15 minutes. RNA preservation is paramount, and the Lysis Buffer SL1 eliminates the need for excessive homogenization while ensuring immediate RNase inactivation, preventing RNA degradation.
Notably, the SPINeasy RNA Kit for Blood eliminates the requirement for prior erythrocyte lysis and leukocyte centrifugation, simplifying the RNA purification process. The inclusion of Fast DNase facilitates efficient on-column DNA digestion, swiftly removing co-purified DNA bound to the silica membrane. Dual washing steps have been optimized to effectively eliminate salts, metabolites, and large cellular components, contributing to a hassle-free and highly time-efficient RNA purification. Following these steps, the elution of high-yield and pure RNA is achieved using RNase-free water. The resulting RNA is well-suited for various downstream applications, including PCR, RT-qPCR amplification, and sequencing.
Performance
Figure 1: SPINeasy RNA Kit for Blood provided higher RNA yield, purity and comparable or higher RNA integrity in comparison to the competitor kits. Frozen whole blood samples (200 µL) were processed using the SPINeasy RNA Kit for Blood and compared with the Competitor M, Z and N kits. Yields were measured with spectrophotometric and fluorometric quantification. Each dot on the plot represents a single extraction.
Figure 2: SPINeasy RNA Kit for Blood delivered the highest yield of amplifiable extracted RNA samples. RNA samples extracted from SPINeasy RNA Kit for Blood and the various competitors were amplified in 15 replicates each for the human GAPDH gene (106bp).
Application Notes
The SPINeasy® RNA Kit for Blood was designed for rapidly isolating high-quality RNA from whole blood samples.
Usage Statement
Research Use Only (RUO)
Key Applications
qPCR amplification
| SKU | 116566050 |
| Application Notes | The SPINeasy® RNA Kit for Blood was designed for rapidly isolating high-quality RNA from whole blood samples. |
| Base Catalog Number | 6566050 |
| Product Line | SPINeasy |
| Components | DNase I (55 µL), DNase I Buffer (3.5 mL), Proteinase K (2.2 mL), Buffer SL1 (22 mL), Buffer SW1 (28 mL), Buffer SW2 (5.6 mL), RNase Free Water (3.5 mL), Column E (50 ea), Collection Tube (50 ea x 2), Elution Tube (50 ea) |
| Pack Size | 50 Preps |
| Personal Protective Equipment | Safety Goggles/Faceshield; Gloves |
| Sample Type | Blood |
| Storage | Store at Room Temperature (15-25°C), Dry environment. |
| Usage Statement | Research Use Only (RUO) |
