The composition of fecal samples is largely dependent on diet which includes fibers, undigested particles, bilirubin, complex polysaccharides, and lipids. Those compounds impair the sample homogenization, decrease both the quantity and quality of the extracted DNA. The SPINeasy® RNA Kit for Feces integrates our 1) state of art bead beating technology, 2) a lysis chemistry compatible with the widest range of samples and 3) our proprietary inhibitor removal expertise. A new technology of selective binding of RNA along with our FastDNAse I treatment allows the complete removal of DNA within 1-2 minutes, further reducing the processing time.
The SPINeasy® RNA Kit for Feces delivers high yields of pure RNA without detectable DNA contamination in less than 20 min, making it particularly suitable for downstream applications, such as qPCR and RT-qPCR without the need of further inhibitor/DNA removal steps.
Performance
The SPINeasy® RNA Kit for Feces has been developed to handle a wide variety of feces samples. When tested on various samples, the SPINeasy® RNA Kit for Feces delivered higher yield, optimal 260/280 and 260/230 ratio and similar or better integrity than competitor Q kit indicating a high extraction performance across a wide range of samples (Figure 1).
Unlike competitor Q kit, no cross-DNA contamination was observed in RNA samples extracted with the SPINeasy® RNA Kit for Feces. As shown in Agilent virtual gel (Figure 1, red rectangle) or agarose gel profile (Figure 2), some RNA samples extracted with the competitor Q kit contained DNA contamination; this highlights the outstanding performance of the SPINeasy® RNA Kit for Feces as compared to other similar commercial kit on the market.
Application Notes
The SPINeasy® RNA Kit for Feces delivers high yields of pure RNA without detectable DNA contamination in less than 20 min, making it particularly suitable for downstream applications, such as qPCR and RT-qPCR without the need of further inhibitor/DNA removal steps.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Genomic, Molecular Biology, PCR, qPCR
| SKU | 116556050 |
| Application Notes | The SPINeasy® RNA Kit for Feces delivers high yields of pure RNA without detectable DNA contamination in less than 20 min, making it particularly suitable for downstream applications, such as qPCR and RT-qPCR without the need of further inhibitor/DNA removal steps. |
| Base Catalog Number | 6556050 |
| Product Line | SPINeasy |
| Components | Equilibration buffer (12 mL), Lysing Matrix YB (50 ea), Phenol: chloroform: isoamyl alcohol (6 mL), Buffer FR1 (40 mL), Buffer FR2 (15 mL), Buffer FR3 (48 mL), Buffer FR4 (72 mL), Buffer FR5 (36 mL), RNase-free water (10 mL), FastDNase I enzyme (0.24 mL), FastDNase I buffer (2 mL), RNA Column (50 ea), 2 mL Tubes (100 ea), Collection Tubes (150 ea), Elution Tubes (50 ea) |
| Pack Size | 50 Preps |
| Personal Protective Equipment | Safety Goggles/Faceshield; Gloves |
| Sample Type | Feces |
| Storage | Store at Room Temperature (15-25°C), Dry environment |
| Usage Statement | Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
