Blood samples serve diverse purposes in disease diagnosis, genetic research, and addressing conditions like disorders and pregnancy complications. Efficient DNA or RNA extraction is imperative, especially when simultaneous extraction is required. Optimized for simultaneous extraction of DNA and RNA from whole blood samples, the SPINeasy® DNA/RNA Kit for Blood addresses challenges associated with inhibitors and low concentrations.
The SPINeasy® DNA/RNA Kit for Blood is a high-performance DNA/RNA extraction kit that is based on silica-membrane spin-column technology. This kit enables simultaneous isolation of genomic DNA and RNA from whole blood preserved in different anticoagulants, in less than 15 minutes. The Lysis Buffer SL1 pairing with minute amount of solvent omits the need of excessive homogenization and enables efficient separation of nucleic acid from the organic impurities fraction consisting of hemoglobin, immunoglobulin, and other blood proteins. The SPINeasy® DNA/RNA kit for Blood enables co-purification of DNA or RNA via two distinct columns from the same sample, making it hassle-free and highly time-efficient, without any sample bias concern. Furthermore, neither DNase nor RNase A is used in the purification process, hence enabling a maximum recovery of DNA and RNA yield. The simplified washing steps largely remove salts, metabolites, and macromolecular cellular components. The extracted DNA and RNA (with high yield and purity) are compatible with downstream applications such as PCR amplification.
Performance
Figure 1. The SPINeasy® DNA/RNA Kit for Blood provides higher yield of nucleic acid and comparable purity. Frozen whole blood sample (200 µL) were processed using the SPINeasy® DNA/RNA Kit for Blood, Competitor Z, and Competitor M kits. Yields were measured by spectrophotometric and fluorometric quantification. Each dot on the plot represents a single extraction.
Figure 2. The SPINeasy® DNA/RNA Kit for Blood gives good quality DNA and RNA. The virtual gels from Agilent 4150 TapeStation analyses were displayed along with the DNA integrity (DIN) and RNA integrity (RIN) values. Higher values, nearing 10, signify intact nucleic acids with minimal degradation. DIN and RIN values exceeding 8 and 7, respectively, indicating the presence of high-quality DNA/RNA suitable for subsequent applications. Each dot of the plot represents a sample.
Figure 3. The SPINeasy® DNA/RNA Kit for Blood delivers high quality DNA and RNA which are fully compatible with downstream qPCR and RT-qPCR, respectively. Both DNA and RNA samples extracted from SPINeasy® DNA/RNA kit for Blood and the various competitors were amplified in 20 replicates (each for the human GAPDH gene (106bp)).
Application Notes
The SPINeasy® DNA/RNA Kit for Blood was designed for the simultaneous isolation of DNA and RNA from the same blood sample.
Usage Statement
Research Use Only (RUO)
Key Applications
PCR amplification
| SKU | 116582050 |
| Application Notes | The SPINeasy® DNA/RNA Kit for Blood was designed for the simultaneous isolation of DNA and RNA from the same blood sample. |
| Base Catalog Number | 6582050 |
| Product Line | SPINeasy |
| Components | Buffer SB1 (11 mL), SB2 (32 mL), SB3 (11 mL), SB4 (10 mL), RNase-free water (11 mL), Equilibration Buffer (12 mL), DNA Column (50 ea), RNA Column (50 ea), DNA Collection Tube (50 ea x 2), RNA Collection Tube (50 ea x 2), DNA Elution tube (50 ea), RNA Elution tube (50 ea) |
| Pack Size | 50 Preps |
| Personal Protective Equipment | Safety Goggles/Faceshield; Gloves |
| Sample Type | Blood |
| Storage | Store at Room Temperature (15-25°C), Dry environment. |
| Usage Statement | Research Use Only (RUO) |
