The SPINeasy RNA Kit for Soil is a high-performance RNA extraction kit utilizing silica-membrane spin-column technology. This kit allows for the efficient isolation of RNA from various soil types, including those with high humic acid content, heavy contaminants, compost, gardening soil, and low-biomass soils. The extraction process can be completed in under an hour with minimal RNA degradation, and the use of hazardous chemicals like phenol or chloroform is unnecessary. Our specially formulated inhibitor removal technology effectively handles soil samples containing humic acids, heavy metals, and other aromatic compounds, ensuring accurate PCR results. RNA extracted with this kit demonstrates high integrity and purity, suitable for downstream applications such as reverse transcription, real-time PCR, and sequencing.
Figure 1: Comparison of RNA extracted from High, Medium and Low Biomass Soils using SPINeasy RNA Kit for Soil, Competitors Q and M. 200 mg of High Biomass, 250 mg of Medium Biomass soil, and 500 mg of Low Biomass Soil were processed. The yield was determined using QuantiFluor® RNA System (Promega). A260/280 and A260/230 ratios were determined using a Spectrophotometer. The virtual gel image and RNA integrity Number (RIN, in yellow or red) were analyzed using Agilent Tapestation 4150.
Figure 2: Amplifiability of RNA extracted with SPINeasy RNA Kit for Soil. Comparison of threshold cycles (Ct) of Reverse Transcription–qPCR when amplification was performed using RNA extracted from SPINeasy RNA Kit for Soil, Competitors Q and M. High Biomass soil (50 ng), Medium Biomass Soil (50 ng) and Low Biomass Soil (30 ng) were used. Targets were amplified with SYBR green technology.
Application Notes
The SPINeasy RNA Kit for Soil was designed for the isolation of RNA from the soil samples.
Usage Statement
Research Use Only (RUO)
Key Applications
reverse transcription, real-time PCR, sequencing
| SKU | 1165850-CF |
| Application Notes | The SPINeasy RNA Kit for Soil was designed for the isolation of RNA from the soil samples. |
| Base Catalog Number | 6585050/6585000 |
| Product Line | SPINeasy |
| Components | Equilibration Buffer R (12 mL), Buffer SR1A (44 mL), Buffer SR1B (8.5 mL), Buffer SR2 (20 mL), Buffer SR3 (44 mL), Buffer SR4 (40 mL), Buffer SR5 (40 mL), Buffer SR6 (2 x 40 mL), DNase I Buffer (4 mL), DNase I (275 µL), RNase-free Water (6 mL), Lysing Matrix YB (50 x 2 mL), Column S (50 ea), Collection tube (2 x 50 ea), and Elution tube (50 ea) |
| Personal Protective Equipment | Safety Goggles/Faceshield; Gloves |
| Sample Type | Soil |
| Storage | Store at Room Temperature (15-25°C), Dry environment. |
| Usage Statement | Research Use Only (RUO) |
