Difficulty in RNA extraction from Mycobacterium
Like any RNA, the RNA in Mycobacterium is typically unstable and prone to degradation. Furthermore, the high lipid content in cell wall increases the difficulty of RNA extraction from Mycobacterium.
Overview
Sample Name:
Mycobacterium smegmatis
Sample Type:
Mycobacterium culture
Challenge :
RNA extraction from Mycobacterium smegmatis was unsuccessful.
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SPINeasy RNA Kit for Bacteria (with Lysing Matrix),50 preps
Quick and convenient purification of total RNA from gram-positive and gram-negative bacteria using spin column technology.
Methods
Sample was prepared in order of protocol in accordance to product guidelines found here.
Based on MP company’s technical advice, we optimized experiment conditions.
Outcome
Trial results data
Electrophoresis chart
The extracted RNA was evaluated in a Nanodrop ND-1000 spectrophotometer.
The acceptable ranges of absorbance ratios at 260/280 and 260/230 were 1.8-2.0 and 1.8-2.2 respectively
Conclusion
Key takeaways:
Based on MP company’s technical advice, we optimized the experiment conditions, and both the RNA yield and purity were improved. The RNA extracted from the Mycobacterium smegmatis can meet downstream experiment requirements. The researcher was satisfied with the results and has already made SPINeasyTM RNA Kit for Bacteria as his choice for future studies.
Looking To Try Our Extraction Kits?MP Biomedicals is now offering Free Trial Pack Application for the SPINeasy ® Tissue and Bacteria w/ Lysing Matrix (5 Preps) to any valid research scientists. |
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